anti-NEK11 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-NEK11 antibody; NEK11;
The NEK11 n/a (Catalog #MBS647920) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NEK11 (Serine/Threonine-protein Kinase Nek11, Never in Mitosis A-related Kinase 11, NimA-related Protein Kinase 11, FLJ23495) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s NEK11 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the NEK11 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:
MLKFQEAAKC VSGSTAISTY PKTLIARRYV LQQKLGSGSF GTVYLVSDKK AKRGEELKVL KEISVGELNP NETVQANLEA QLLSKLDHPA IVKFHASFVE QDNFCIITEY CEGRDLDDKI QEYKQAGKIF PENQIIEWFI QLLLGVDYMH ERRILHRDLK SKNVFLKNNL LKIGDFGVSR LLMGSCDLAT TLTGTPHYMS PEALKHQGYD TKSDIWSLAC ILYEMCCMNH AFAGSNFLSI VLKIVEGDTP SLPERYPKEL NAIMESMLNK NPSLRPSAIE ILKIPYLDEQ LQNLMCRYSE MTLEDKNLDC QKEAAHIINA MQKRIHLQTL RALSEVQKMT PRERMRLRKL QAADEKARKL KKIVEEKYEE NSKRMQELRS RNFQQLSVDV LHEKTHLKGM EEKEEQPEGR LSCSPQDEDE ERWQGREEES DEPTLENLPE SQPIPSMDLH ELESIVEDAT SDLGYHGDCN LISLDEYWKN EK.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NEK11 (Serine/Threonine-protein Kinase Nek11, Never in Mitosis A-related Kinase 11, NimA-related Protein Kinase 11, FLJ23495) with the following immunoassay(s):
Western Blot (WB) (Western Blot detection against Immunogen (79.13kD).)
Western Blot (WB) (Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody.|Lane 1: NEK11 transfected lysate (55.5kD).|Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3ug/ml].)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data (Detection limit for recombinant GST tagged NEK11 is 0.1ng/ml as a capture antibody.)
Western Blot (WB) (NEK11 monoclonal antibody, Western Blot analysis of NEK11 expression in HL-60.)
Western Blot (WB) (NEK11 monoclonal antibody. Western Blot analysis of NEK11 expression in A-431.)
Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.
Immunogen: Full length recombinant corresponding to aa1-483 from human NEK11 (AAH28587) with GST tag. MW of the GST tag alone is 26kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NEK11 are readily searchable from our website. Different antibodies against the same target such as NEK11 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.