anti-Nesprin antibody product blog
Tags: Antibody; Monoclonal Antibody; Nesprin; anti-Nesprin antibody;
The Nesprin n/a (Catalog #MBS570211) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Nesprin reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Nesprin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (frozen).Nsp3 is useful for immunohistochemistry on frozen tissues and cell cultures fixed with methanol, or 4% paraformaldehyde combined with a permeabilization step of 15\' in 0.1% Triton in PBS. The antibody is also suited for immunoblotting. Optimal antibody dilution should be determined by titRation. Recommended range is 1:100 - 1:1000 for immunohistochemistry with FITC-conjugated secondary antibodies or avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagents. Researchers should empirically determine the suitability of the Nesprin n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Nesprin n/a product has the following accession number(s) (GI #167878104) (NCBI Accession #EDS41487.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse anti Nesprin with the following immunoassay(s):
Testing Data (Immunofluorescence staining of a cultured mouse embryonic fibroblast with Nsp3 for nesprin-3a (green), and counter-staining of nuclear DNA with DAPI (blue). )
Nesprin-3, is a member of the nesprin family and is a transmembrane protein of the outer nuclear membrane (ONM). Like nesprin-1 and nesprin-2, nesprin-3 also contains a COOH-terminal klarsicht/ANC-1/syne (KASH) domain and a series of spectrin repeats. In Humans, the full length nesprin-1 and nesprin-2 are giant proteins, containing both an actin-binding domain resulting in molecular masses of respectively 976 and 764 kDa. These proteins are therefore suggested to be involved in nuclear positioning. Nesprin-3, in contrast to nesprin-1 and nesprin-2, lacks the actin-binding domain and cannot bind directly to the cytoskeleton. Nesprin-3 has two isoforms, nesprin-3a and nesprin-3b, which differ in the N-terminus. The Nsp3 monoclonal antibody reacts specifically with nesprin-3a. The N-terminus of nesprin-3a, but not that of nesprin-3b interacts with the cytoskeletal crosslinker protein plectin. Plectin influences the structural organization and integrity of the cytoskeleton and is thought to assist in the mechanical strengthening of the cells. Plectin binds to nesprin-3a and to the intermediate filaments through its C-terminus, suggesting that the interaction between nesprin-3a and plectin links the nucleus to the intermediate filament system. Nesprin-3, like nesprin-1 and nesprin-2 is localized in the nuclear envelope. This localization is due to its interaction with SUN proteins, both SUN-1 and SUN-2, present in the inner nuclear membrane.