anti-Neurofilament L, 68kD antibody product blog
Tags: Antibody; Monoclonal Antibody; Neurofilament L, 68kD; anti-Neurofilament L, 68kD antibody;
The Neurofilament L, 68kD n/a (Catalog #MBS608850) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament L, 68kD (NF-L) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Neurofilament L, 68kD can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: ELISA: 0.1-0.5ug/ml
Immunohistochemistry (alcohol and paraformaldehyde-fixed paraffin-embedded or frozen tissue sections): 1-5ug/ml
Western Blot: 0.1-0.5ug/ml
Immunoprecipitation: 2-5ug. Researchers should empirically determine the suitability of the Neurofilament L, 68kD n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Neurofilament L, 68kD (NF-L) with the following immunoassay(s):
Immunofluorescence (IF) (Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded rat brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT. Tissues were then probed with MBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Immunofluorescence (IF) (Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded mouse brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT Tissues were then probed with MBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibod, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Immunohistochemistry (IHC) (Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded human brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT Tissues were then probed withMBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibod, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of the neurofilament light chain showing staining in the filaments of paraffin-embedded rat brain tissue (right) compared to a negative control without MBS608850 (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at RT, washed with ddH2O and PBS, and then probed with MBS608850 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.)
Western Blot (WB) (Western Blot analysis of neurofilament, light chain was performed by loading 25ug of human and mouse brain tissue lysate per well onto a polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked. NF-L was detected at ~70kD using MBS608850 at a dilution of 0.5ug/ml in blocking buffer overnight at 4C, followed by a HRP-labeled secondary antibody for 1 hour at RT and detection with a chemiluminescent substrate.)
Neurofilaments are intermediate (10-12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD). And NF-H (200kD). The neurofilaments are long helical proteins which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, how phosphorylation likely mediates neurofilament function remains unclear. Neurofilament proteins (NFPs) are a macromolecular complex comprised of 3 polypeptides designated as NF-L, NF-M and NF-H. NFPs are found in the perikarya, particularly in neuronal axons throughout the central and peripheral nervous system. Since NFPs are major structural proteins and biochemically quite stable, antibodies to NFPs are useful probes in studies of neuronal expression, morphology, connectivity and pathology. The presence or absence of NFP in a variety of nervous system or neuroendocrine tumors can provide useful information about the original cell type of the tumor. In addition, the normal NFP staining pattern is altered in a variety of human diseases including Alzheimer\'s disease, diffuse cortical Lewy body disease, Parkinson\'s disease and amyotrophic lateral sclerosis (Lou Gehrig\' disease).