anti-NF-KAPPAB1 P105/P50 antibody product blog
Tags: Antibody; NFKB1; Monoclonal Antibody; anti-NF-KAPPAB1 P105/P50 antibody; NF-KAPPAB1 P105/P50;
The NFKB1 nfkb1 (Catalog #MBS475311) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-NFKB1 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s NFKB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:500. Researchers should empirically determine the suitability of the NFKB1 nfkb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NFKB1 nfkb1 product has the following accession number(s) (GI #259155302) (NCBI Accession #NP_001158884.1) (Uniprot Accession #P19838). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-NFKB1 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of NF-kappaB1 p105/p50 in K562, C6, 3T3 and Hela cell lysates using NF-kappaB1 p105/p50 mouse mAb (dilution 1:500).Predicted band size:120, 50kDa.Observed band size:120, 50kDa.)
Entrez Summary: This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].
Immunogen: Recombinant human NF-kappaB1 p105/p50 protein.
Sensitivity: This antibody detects endogenous levels of NF-kappaB1 p105/p50 and does not cross-react with related proteins.
Sequence Similarities: Contains 7 ANK repeats.Contains 1 death domain.Contains 1 RHD (Rel-like) domain. Subunit Structure: Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-p50 complex. Homodimer; component of the NF-kappa-B p50-p50 complex. Component of the NF-kappa-B p105-p50 complex. Component of the NF-kappa-B p50-c-Rel complex. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL. NFKB1/p105 interacts with CFLAR; the interaction inhibits p105 processing into p50. NFKB1/p105 forms a ternary complex with MAP3K8 and TNIP2. Interacts with GSK3B; the interaction prevents processing of p105 to p50. NFKB1/p50 interacts with NFKBIE. NFKB1/p50 interacts with NFKBIZ. Nuclear factor NF-kappa-B p50 subunit interacts with NFKBID (By similarity). Directly interacts with MEN1. Interacts with HIF1AN.
Subcellular Location: Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor.