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anti-NFkappaB p65 antibody product blog

Posted on 2016-02-02 19:09:30 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; NFkappaB p65; anti-NFkappaB p65 antibody;
The NFkappaB p65 rela (Catalog #MBS9400469) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NFkappaB p65 Mouse Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NFkappaB p65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
Western blotting: 1:1000~1:3000
Immunohistochemistry: 1:200. Researchers should empirically determine the suitability of the NFkappaB p65 rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NFkappaB p65 rela product has the following accession number(s) (GI #223468681) (NCBI Accession #NP_001138610.1) (Uniprot Accession #Q04206). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NFkappaB p65 Mouse Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Hela (lane 1), 293T(lane 2) and HepG (lane 3), using diluted at 1:2,000.)
Western Blot (WB) NFkappaB p65.

Western Blot (WB) (Western blot analysis of Mouse Heart (lane 1), Rat Heart (lane 2), Rat Liver (lane 3) and Rat Spleen (lane 4), using diluted at 1:3,000.)
Western Blot (WB) NFkappaB p65.

Immunohistochemistry (IHC) (IHC staining of mouse hippocampus tissue with NFkB p65 mouse mAb(14H2) diluted at 1:200.)
Immunohistochemistry (IHC) NFkappaB p65.

NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.

Target Name: NFkB p65. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NFkappaB p65 are readily searchable from our website. Different antibodies against the same target such as NFkappaB p65 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Heart, Intestine, Kidney, Liver, Lung, Muscle, Vascular tissues are correlated with this protein. NFkappaB p65 also interacts with the following gene(s): CHUK, CREBBP, EP300, HDAC1, HDAC2, IKBKB, NFKB1, NFKBIA, NFKBIB, UBC.
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