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anti-NGFR antibody product blog

Posted on 2015-11-25 16:26:04 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; NGFR; anti-NGFR antibody;
The NGFR ngfr (Catalog #MBS9200505) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NGFR Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s NGFR can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).
IHC~~1:50~100. Researchers should empirically determine the suitability of the NGFR ngfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NGFR ngfr product has the following accession number(s) (GI #4505393) (NCBI Accession #NP_002498.1) (Uniprot Accession #P08138). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NGFR Antibody with the following immunoassay(s):
Immunofluorescence (IF) (Fluorescent confocal image of SY5Y cells stained with NGFR antibody SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9200505 NGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).)
Immunofluorescence (IF) NGFR.

Western Blot (WB) (Western blot analysis of NGFR Antibody in mouse heart tissue lysates (35ug/lane).NGFR (arrow) was detected using the purified Mab.(1:1000))
Western Blot (WB) NGFR.

Immunohistochemistry (IHC) (NGFR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NGFR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC) NGFR.

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NGFR are readily searchable from our website. Different antibodies against the same target such as NGFR may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. NGFR also interacts with the following gene(s): BDNF, LINGO1, NGF, NGFRAP1, NTF3, NTRK1, NTRK2, RTN4R, TRAF6.
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