anti-NK-1.1 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD161b/c; anti-NK-1.1 antibody; NK-1.1;
The NK-1.1 klrb1b (Catalog #MBS212065) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD161b/c can be used in a range of immunoassay formats including, but not limited to, Cytotoxic Assays (CT), Flow cytometry (FC/FACS), Immunoprecipitation (IP).Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Cytotoxic Assays: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml
Flow Cytometry: Minimum Dilution: 5ug/ml; Maximum Dilution: 10ug/ml. Researchers should empirically determine the suitability of the NK-1.1 klrb1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NK-1.1 klrb1b product has the following accession number(s) (GI #128356) (NCBI Accession #P27812.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI MOUSE NK-1.1 ANTIGEN with the following immunoassay(s):
Testing Data (Staining of New Zealand Black mouse peripheral blood lymphocytes with Mouse anti Mouse NK Cells:RPE (MBS213569))
Mouse anti Mouse NK-1.1 Antigen antibody, clone PK136 (clone PK136) recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc. Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. Mouse anti Mouse NK-1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti Mouse NK-1.1 Antigen antibody, clone PK136 has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein A. Immunogen: Spleen and bone marrow cells from CE mice
Fusion Partners: Spleen cells from immunised (C3H x BALB/c) FI Hybrid were fused with cells of the Sp2/0 - Ag14 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse.