anti-NK1.1 antibody product blog
Tags: Antibody; Monoclonal Antibody; NK1.1; anti-NK1.1 antibody;
The NK1.1 n/a (Catalog #MBS832545) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s NK1.1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).FC: 5 ul (0.125 ug)/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Researchers should empirically determine the suitability of the NK1.1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NK1.1 antibody (FITC) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of C57Bl/6 splenocytes with CD49b antibody (eFluor 450) and 0.25 ug of Mouse IgG2a K Isotype Control (FITC) (left) or 0.25 ug of NK1.1 antibody (FITC) (right). Cells in the lymphocyte gate were used for analysis.)
Mouse monoclonal NK1.1 antibody (FITC).
Biological Significance: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 14-5971 should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells. Tag/Conjugate: FITC. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NK1.1 are readily searchable from our website. Different antibodies against the same target such as NK1.1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.