anti-NME5 antibody product blog
Tags: Antibody; Monoclonal Antibody; NME5; anti-NME5 antibody;
The NME5 nme5 (Catalog #MBS6010993) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NME5 (Nucleoside Diphosphate Kinase Homolog 5, NDK-H 5, NDP Kinase Homolog 5, Inhibitor of p53-induced Apoptosis-beta, IPIA-beta, Testis-specific nm23 Homolog, nm23-H5) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s NME5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP).Suitable for use in ELISA, Western Blot and Immunoprecipitation. Researchers should empirically determine the suitability of the NME5 nme5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NME5 nme5 product has the following accession number(s) (GI #4505413) (NCBI Accession #NP_003542.1) (Uniprot Accession #P56597). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
DLRNALHGSN DFAAAEREIR FMFPEVIVEP IPIGQAAKDY LNLHIMPTLL EGLTELCKQK PADPLIWLAD WLLKNNPNKP KLCHHPIVEE PY.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NME5 (Nucleoside Diphosphate Kinase Homolog 5, NDK-H 5, NDP Kinase Homolog 5, Inhibitor of p53-induced Apoptosis-beta, IPIA-beta, Testis-specific nm23 Homolog, nm23-H5) with the following immunoassay(s):
Western Blot (WB) (Western Blot detection against Immunogen (35.86kD).)
Immunoprecipitation (IP) (Immunoprecipitation of NME5 transfected lysate using NME5 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with NME5 rabbit polyclonal antibody.)
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Immunogen: Partial recombinant corresponding to aa121-213 from NME5 (NP_003542) with GST tag. MW of the GST tag alone is 26kD.