anti-NOS Clone 5D5-H7 antibody product blog
Tags: Antibody; Monoclonal Antibody; NOS Clone 5D5-H7; anti-NOS Clone 5D5-H7 antibody;
The NOS Clone 5D5-H7 n/a (Catalog #MBS350058) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The NOS Clone 5D5-H7 reacts with N/ACrossreactivity Note: No cross reaction with nNOS or eNOS Histochem and ICC staining and may cross-react with other species as described in the data sheet.
The NOS Clone 5D5-H7 n/a product has the following accession number(s) (GI #295410244). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
This IgG fraction of monoclonal antibody 5D5-H7 has been found to stain cells induced to produce iNOS using a dilution of 1.0 ?g/ml. The ability of this monoclonal antibody to bind to iNOS in fixed cells was examined using three different cell lines. DLD-1 (a human colorectal carcinoma cell line). A-172 (a human glioblastoma cell line) and RAW 264.7 (a mouse macrophage cell line). The cells were cultured for 2 days in normal medium and then induced to produce iNOS by treatment for 16 hours with a cytokine/LPS mixture. Following the treatment. the cells were washed x 4 and fixed with neutral buffered formalin. They were reacted for 60 minutes with the primary antibody. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescence microscopy. This IgG fraction has also been found to stain freshly fixed tissue and frozen sections by histochemical techniques.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOS Clone 5D5-H7 are readily searchable from our website. Different antibodies against the same target such as NOS Clone 5D5-H7 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein. NOS Clone 5D5-H7 also interacts with the following gene(s): FLNA.