|The NOS2 n/a (Catalog #MBS476305) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOS2 Mouse mAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s NOS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the NOS2 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-NOS2 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot analysis using NOS2 mAb against human NOS2 (AA). (Applaction:Transfection of recombinant protein))
Western Blot (WB) (Western blot analysis using NOS2 mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of MCF-7 cells using NOS2 mouse mAb (green) and negative control (red).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NOS2 mouse mAb with DAB staining.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NOS2 mouse mAb with DAB staining.)
Entrez Summary: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Immunogen: Purified recombinant fragment of human NOS2 expressed in E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOS2 are readily searchable from our website. Different antibodies against the same target such as NOS2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.