anti-NOTCH1 antibody product blog
Tags: Antibody; Monoclonal Antibody; NOTCH1; anti-NOTCH1 antibody; NOTCH 1;
The NOTCH1 notch1 (Catalog #MBS832889) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Notch 1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).FC: <= 0.125 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the NOTCH1 notch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NOTCH1 notch1 product has the following accession number(s) (GI #11275980) (NCBI Accession #AAG33848.1) (Uniprot Accession #P46531). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Notch 1 antibody (PE) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of C57BL/6 thymocytes with 0.06 ug of Mouse IgG1 kappa Isotype Control (PE) (open histogram) or 0.125 ug of Notch1 antibody (PE) (filled histogram). CD4-CD8- thymocytes were used for analysis.)
Mouse monoclonal Notch 1 antibody (PE).
Biological Significance: The Notch family of transmembrane receptors controls cell-fate decisions during the development of many organs in a wide variety of species. After binding its ligand, the Notch receptor is cleaved in its transmembrane domain, and the resulting intracellular domain dissociates from the membrane and translocates to the nucleus, where it is able to suppress the expression of lineage-specific genes by interacting with transcriptional repressors. The mN1A antibody reacts with the intracellular domain of mouse and human Notch1, but not with Notch2, 3, or 4. The mN1A antibody has a low affinity for the full-length forms of Notch1. In the mouse, Notch mRNA is expressed in mouse hematopoietic cells of the fetal liver and adult thymus and bone marrow. In the thymus, Notch1 protein is detected in CD4-CD8- and CD4-CD8+ thymocytes. Studies of Notch1-transgenic cells and Notch1-null mice indicate that the receptor is involved in the regulation of lymphopoiesis and myelopoiesis. Tag/Conjugate: PE. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOTCH1 are readily searchable from our website. Different antibodies against the same target such as NOTCH1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Bone Marrow, Brain, Embryonic Tissue, Heart, Liver, Muscle, Thymus, Vascular tissues are correlated with this protein. Breast Neoplasms, Cell Transformation, Neoplastic, Congenital Abnormalities, Inflammation, Leukemia, T-Cell, Liver Diseases, Lymphoma, Neoplasm Metastasis, Nervous System Diseases, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma are some of the diseases may be linked to Notch 1 antibody (PE). NOTCH1 also interacts with the following gene(s): ADAM17, DTX1, FBXW7, HEY1, HEY2, MAML1, NUMB, PSEN1, RBPJ. The following patways have been known to be associated with this gene.