anti-NQO1 antibody product blog
Tags: Antibody; Monoclonal Antibody; NQO1; anti-NQO1 antibody;
The NQO1 nqo1 (Catalog #MBS476236) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-NQO1 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s NQO1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the NQO1 nqo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The NQO1 nqo1 product has the following accession number(s) (GI #4505415) (NCBI Accession #NP_000894.1) (Uniprot Accession #P15559). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-NQO1 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from MCF7 and Hela cell lysates using NQO1 mouse mAb (1:1000 diluted).Predicted band size:31KDa.Observed band size:31KDa.)
Western Blot (WB) (Western blot analysis using NQO1 mouse mAb against A549 (1), Hela (2), MCF-7 (3) and HepG2 (4) cell lysate.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of HepG2 cells using NQO1 mouse mAb (green) and negative control (red).)
Entrez Summary: This gene is a member of the NAD (P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This proteins enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimers disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniPort Summary Function: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Immunogen: Purified recombinant fragment of human NQO1 expressed in E Coli.
Sequence Similarities: Belongs to the NAD (P)H dehydrogenase (quinone) family. Subunit Structure: Homodimer.
Subcellular Location: Cytoplasm. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NQO1 are readily searchable from our website. Different antibodies against the same target such as NQO1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.