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anti-NR3C1 antibody product blog

Posted on 2015-09-11 04:08:41 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-NR3C1 antibody; NR3C1; NR3C1/Glucocorticoid Receptor;
The NR3C1 nr3c1 (Catalog #MBS249109) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 6E6] (IgG1) to Human NR3C1/Glucocorticoid Receptor reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s NR3C1/Glucocorticoid Receptor can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA).
ELISA (1:10000), Flo (1:200 - 1:400), ICC (1:200 - 1:1000), IF (1:1000), IHC-P (1:200), WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the NR3C1 nr3c1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NR3C1 nr3c1 product has the following accession number(s) (GI #4504133) (NCBI Accession #NP_000167.1) (Uniprot Accession #P04150). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 6E6] (IgG1) to Human NR3C1/Glucocorticoid Receptor with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-NR3C1/Glucocorticoid Receptor antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC) NR3C1.

Immunofluorescence (IF) (Immunofluorescence of PC-2 cells using NR3C1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF) NR3C1.

Flow Cytometry (FC/FACS) (Flow cytometry of K562 cells using NR3C1 mouse mAb (green) and negative control (purple).)
Flow Cytometry (FC/FACS) NR3C1.

Western Blot (WB) (Western blot of NR3C1 mouse mAb against HeLa cell lysate.)
Western Blot (WB) NR3C1.

ELISA (EIA) (Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)

The glucocorticoid receptor (GR), a NR3 Steroid Receptor, mediates the physiological activities of glucocorticoids that modulate development, homeostasis, stress, inflammatory, and immunologic responses. GR has been shown to mediate negative-feedback signals of elevated glucocorticoid levels; impaired GR signaling is a key mechanism in the pathogenesis of stress-related psychiatric disorders such as depression and anxiety.

Target Species: Human
Immunogen Description: Purified recombinant fragment of human NR3C1 expressed in E Coli. Immunogen Type: Recombinant protein
Immunogen: NR3C1/Glucocorticoid Receptor antibody was raised against purified recombinant fragment of human NR3C1 expressed in E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NR3C1 are readily searchable from our website. Different antibodies against the same target such as NR3C1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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