anti-Nrcam antibody product blog
Tags: Antibody; Monoclonal Antibody; NRCAM; anti-Nrcam antibody;
The Nrcam nrcam (Catalog #MBS802548) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NrCAM Antibody: ATTO 488 reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NrCAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).1:1000 WB. Researchers should empirically determine the suitability of the Nrcam nrcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Nrcam nrcam product has the following accession number(s) (GI #225637535) (NCBI Accession #NP_001139503.1) (Uniprot Accession #Q810U4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NrCAM Antibody: ATTO 488 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51. Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.)
Background Info: Detects a 160 kDa protein.
Scientific Background: Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.
Conjugate: ATTO 488. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Nrcam are readily searchable from our website. Different antibodies against the same target such as Nrcam may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.