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anti-NRF1 antibody product blog

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Posted on 2016-04-23 23:48:49 by mybiosource_staff
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Tags: Antibody; NRF1; anti-NRF1 antibody; Monoclonal Antibody;
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The NRF1 nrf1 (Catalog #MBS9128975) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NRF1 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NRF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS).
WB: 1:500 - 1:2000, IHC: 1:50 - 1:100, ICC: 1:50 - 1:100, IP: 1:20 - 1:50, FC: 1:20 - 1:50. Researchers should empirically determine the suitability of the NRF1 nrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NRF1 nrf1 product has the following accession number(s) (GI #93141039) (NCBI Accession #NP_001035199.1) (Uniprot Accession #Q16656). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NRF1 Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NRF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (MBS128212) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.)
Western Blot (WB) NRF1.

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Immunogen: Recombinant protein of human NRF1. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NRF1 are readily searchable from our website. Different antibodies against the same target such as NRF1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Embryonic Tissue, Eye, Heart, Kidney, Liver, Lung, Nerve, Skin tissues are correlated with this protein.
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