anti-NT5E antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-NT5E antibody; NT5E; CD73 (NT5E);
The NT5E nt5e (Catalog #MBS9200435) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD73 (NT5E) Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD73 (NT5E) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).WB~~1:5000. Researchers should empirically determine the suitability of the NT5E nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NT5E nt5e product has the following accession number(s) (GI #325651886) (NCBI Accession #NP_001191742.1) (Uniprot Accession #P21589). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD73 (NT5E) Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (Anti-CD73 (NT5E) Antibody (C-term) at 1:4000 dilution + MDA-MB-231 whole cell lysates
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 63 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Anti-CD73 (NT5E) Antibody (C-term) at 1:2000 dilution + WiDr whole cell lysates
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 63 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (CD73 (NT5E) Antibody (C-term) western blot analysis in MDA-MB-231,WiDr cell line lysates (35ug/lane).This demonstrates the CD73 (NT5E) antibody detected the CD73 (NT5E) protein (arrow).)
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Antigen Type: Recombinant Protein
Crown Antibody: Yes. Antigen Source: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NT5E are readily searchable from our website. Different antibodies against the same target such as NT5E may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. NT5E also interacts with the following gene(s): ADK, ENPP1, ENTPD1, ENTPD3, HIF1A. Blood, Bone, Bone Marrow, Brain, Connective Tissue, Ganglia, Kidney, Mouth, Muscle, Vascular tissues are correlated with this protein. Brain Diseases, Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Heart Diseases, Inflammation, Kidney Diseases, Necrosis, Nervous System Diseases, Pain are some of the diseases may be linked to CD73 (NT5E) Antibody (C-term).