anti-Nucleoprotein Interactor-1, hSRP-1 antibody product blog
Tags: Antibody; Monoclonal Antibody; Nucleoprotein Interactor-1, hSRP-1; anti-Nucleoprotein Interactor-1, hSRP-1 antibody;
The Nucleoprotein Interactor-1, hSRP-1 n/a (Catalog #MBS601602) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nucleoprotein Interactor-1, hSRP-1 (NPI-1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Nucleoprotein Interactor-1, hSRP-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF).Suitable for use in Immunoprecipitation, Immunofluorescence, ELISA and Western Blot. Other applications have not been tested
Dilution: Immunoprecipitation (native): 5-10ug
Immunofluorescence: 5-10ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml. Researchers should empirically determine the suitability of the Nucleoprotein Interactor-1, hSRP-1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. NPI-1 (nucleoprotein interactor-1, hSRP-1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein\'s nuclear localization signal. Both subunits of karyopherin are required for protein docking at the NPC; the alpha subunit binds the substrate and the beta subunit acts as an adaptor. Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin beta and promotes the release of both subunits from the docking site. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC.
Immunogen: GST fusion protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Nucleoprotein Interactor-1, hSRP-1 are readily searchable from our website. Different antibodies against the same target such as Nucleoprotein Interactor-1, hSRP-1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.