|The OCRL ocrl (Catalog #MBS801341) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The INPP5F Antibody: ATTO 594 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s INPP5F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
1:1000 WB. Researchers should empirically determine the suitability of the OCRL ocrl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The OCRL ocrl product has the following accession number(s) (GI #13325072) (NCBI Accession #NP_000267.2) (Uniprot Accession #Q01968). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the INPP5F Antibody: ATTO 594 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Monkey COS cells transfected with GFP-tagged OCRL showing detection of ~100 kDa INPP5F protein using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged OCRL. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.)
Background Info: Detects a 100 kDa protein. Cross-reacts with INPP5b.
Scientific Background: Inositol polyphosphate 5-phosphatase OCRL-1 (INPP5F/OCRL) is also called Lowe oculocerebrorenal syndrome protein. INPP5F is a phosphotase that converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate, as well as inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. ORCL is involved in primary cilia assembly and may also have a role in lysosomal membrane trafficking. ORCL is expressed in brain, skeletal muscle, heart, kidney, lung, placenta, fibroblasts, retina and the retinal pigment epithelium. INPP5F defects can cause Lowe oculocerebrorenal syndrome (OCRL) and Dent disease type 2 (DD2).
Conjugate: ATTO 594. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing OCRL are readily searchable from our website. Different antibodies against the same target such as OCRL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.