anti-ORAI3 antibody product blog
Tags: Antibody; Monoclonal Antibody; ORAI3; anti-ORAI3 antibody;
The ORAI3 orai3 (Catalog #MBS9400189) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s ORAI3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the ORAI3 orai3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The ORAI3 orai3 product has the following accession number(s) (GI #126364340) (NCBI Accession #BAF47907.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ORAI3 Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of ORAI3 in rat spleen lysate with ORAI3 antibody at 2 ug/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of ORAI3 in rat spleen tissue with ORAI3 antibody at 2.5 ug/mL.)
Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.
Immunogen Type: Peptide
Immunogen Description: A 19 amino acid peptide from near the carboxy terminus of human ORAI3. Target Name: ORAI3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ORAI3 are readily searchable from our website. Different antibodies against the same target such as ORAI3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. ORAI3 also interacts with the following gene(s): Stim1, Stim2, Trpc1, Trpc3, Trpc4, Trpc5, Trpc6.