|The PARP parp1 (Catalog #MBS840142) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PARP Antibody (Clone 10H) reacts with All species and may cross-react with other species as described in the data sheet. MyBioSource\'s PARP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC).
Immunocytochemistry : 5-20 mug/ml; Western Blot (10 mug/ml using colorimetric methods; <2 mug/ml for ECL); Immunoprecipitation and ELISA. Researchers should empirically determine the suitability of the PARP parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PARP parp1 product has the following accession number(s) (GI #156523968) (NCBI Accession #NP_001609). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PARP Antibody (Clone 10H) with the following immunoassay(s):
Background: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP\'s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Positive Control: Tested by ICC in rat liver tissues. Handling: The antibody solution should be gently mixed before use. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PARP are readily searchable from our website. Different antibodies against the same target such as PARP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Brain, Heart, Kidney, Liver, Lung, Muscle, Nerve, Prostate, Vascular tissues are correlated with this protein. PARP also interacts with the following gene(s): CASP3, CASP7, PARG, PCNA, TP53, UBC, WRN, XRCC1, XRCC5, XRCC6. Brain Ischemia, Breast Neoplasms, Heart Diseases, Inflammation, Liver Diseases, Liver Neoplasms, Lung Neoplasms, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to PARP Antibody (Clone 10H).