|The PAX-8 pax-8 (Catalog #MBS346026) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PAX-8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PAX-8 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin.
Dilution Range: 1:100 - 1:200. Researchers should empirically determine the suitability of the PAX-8 pax-8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PAX-8 pax-8 product has the following accession number(s) (GI #1850829) (NCBI Accession #CAA67903.1) (Uniprot Accession #Q99573). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PAX-8 Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (IHC (paraffin) of human thyroid gland using PAX-8 Antibody)
PAX-8 is a transcription factor in the paired box (PAX) family, whose mainly functions as important regulators in early development, especially in thyroid follicular cells and thyroid specific genes. Mutations in PAX-8 could lead to thyroid follicular carcinoams, atypical thyroid adenomas, and thyroid dysgenesis. Studies have associated PAX-8 expression with renal carcinoma, nephroblastoma, and seminoma, and have indicated PAX-8 as an useful marker for renal epithelial tumors, ovarian cancer, and for differential diagnoses in lung and neck tumors.
Buffer: Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3. IHC Positive Control: Ovarian, Thyroid, and Renal Cell Carcinomas
IHC Suggested Protocol: 1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PAX-8 are readily searchable from our website. Different antibodies against the same target such as PAX-8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.