anti-PAX8 antibody product blog
Tags: Antibody; Monoclonal Antibody; PAX8; anti-PAX8 antibody;
The PAX8 pax8 (Catalog #MBS668451) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to PAX8 (Clone: ABM5F33) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PAX8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB).WB: 2-4 ug/ml
Flow Cytometry: 0.5-1 ug/10^6. Researchers should empirically determine the suitability of the PAX8 pax8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PAX8 pax8 product has the following accession number(s) (GI #215273928) (NCBI Accession #Q06710.2) (Uniprot Accession #Q06710). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Monoclonal Antibody to PAX8 (Clone: ABM5F33) with the following immunoassay(s):
Western Blot (WB) (Fig-1: Western blot analysis of PAX8. Anti-PAX8 antibody (Clone: ABM5F33) was used at 0.5 ug/ml on recombinant and 2 ug/ml on Raji lysate.)
Flow Cytometry (FC/FACS) (Fig-2: Intracellular flow analysis of Pax8 in Raji cells using 0.5 ug/10^6 cells of antibody (Clone: ABM5F33). Green represents isotype control; red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Immunogen Information: A partial length recombinant protein (a.a 28-261) of PAX8 was used as the immunogen for this antibody. Antigen: PAX8. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PAX8 are readily searchable from our website. Different antibodies against the same target such as PAX8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. PAX8 also interacts with the following gene(s): NKX2-1, PPARG, RB1, RXRA, WT1. Carcinoma, Squamous Cell, Death, Hyperplasia, Kidney Diseases, Lung Diseases, Necrosis, Neoplasms, Experimental, Pancreatic Neoplasms, Thyroid Diseases, Thyroid cancer, follicular are some of the diseases may be linked to Monoclonal Antibody to PAX8 (Clone: ABM5F33).