anti-PCDH8 antibody product blog
Tags: Antibody; Monoclonal Antibody; Pcdh8; anti-PCDH8 antibody;
The PCDH8 pcdh8 (Catalog #MBS6001723) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PCDH8 (Protocadherin-8, Arcadlin) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PCDH8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the PCDH8 pcdh8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PCDH8 pcdh8 product has the following accession number(s) (GI #14589937) (NCBI Accession #NP_116567.1) (Uniprot Accession #O95206). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
VRYSTFEEDA PGTVIGTLAE DLHMKVSGDT SFRLMKQFNS SLLRVREGDG QLTVGDAGLD RERLCGQAPQ CVLAFDVVSF SQEQFRLVH.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PCDH8 (Protocadherin-8, Arcadlin) with the following immunoassay(s):
Western Blot (WB) (Western Blot detection against Immunogen (35.53kD).)
Western Blot (WB) (PCDH8 monoclonal antibody Western Blot analysis of PCDH8 expression in COLO 320 HSR.)
Western Blot (WB) (Western Blot analysis of PCDH8 expression in transfected 293T cell line by PCDH8 monoclonal antibody.|Lane 1: PCDH8 transfected lysate (113kD).|Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to PCDH8 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
Testing Data (Detection limit for recombinant GST tagged PCDH8 is ~0.1ng/ml as a capture antibody.)
Western Blot (WB) (Western blot analysis of PCDH8 over-expressed 293 cell line, cotransfected with PCDH8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDH8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes an integral membrane protein that is thought to function in cell adhesion in a CNS-specific manner. Unlike classical cadherins, which are generally encoded by 15-17 exons, this gene includes only 3 exons. Notable is the large first exon encoding the extracellular region, including 6 cadherin domains and a transmembrane region. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq].
Immunogen: Partial recombinant corresponding to aa32-121 from human PCDH8 (NP_002581) with GST tag. MW of the GST tag alone is 26kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PCDH8 are readily searchable from our website. Different antibodies against the same target such as PCDH8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.