|The Pcdhgb2 pcdhgb2 (Catalog #MBS800692) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Protocadherin Gamma B2 Antibody: Streptavidin reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Protocadherin Gamma B2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
1:1000 WB. Researchers should empirically determine the suitability of the Pcdhgb2 pcdhgb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Pcdhgb2 pcdhgb2 product has the following accession number(s) (GI #18087735) (NCBI Accession #NP_291053.1) (Uniprot Accession #Q91XX7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Protocadherin Gamma B2 Antibody: Streptavidin with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-B2 showing detection of ~100 kDa Protocadherin Gamma B2 protein using Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody, Clone S148-30 (SMC-452). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-B2. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody (SMC-452) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.)
Background Info: Detects a 100 kDa protein. No cross-reactivity against other Protocadherin Gamma -A, -B or -C proteins.
Scientific Background: Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. The protocadherein gamma cluster consists of three subfamilies (A, B and C). As a member of the gamma subfamily B, PCDHGB2 (Protocadherin gamma B2) is a 931 amino acid protein that is one of 22 proteins encoded by the protocadherin gamma cluster. Typical of gamma protocadherins, PCDHGB2 contains six cadherin motifs and is a type I transmembrane receptor expressed in the central nervous system. With localization to synapses, members of the gamma cluster of protocadherins are essential for neuronal survival. There are two isoforms of PCDHGB2 that are produced as a result of alternative splicing events.
Conjugate: Streptavidin. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Pcdhgb2 are readily searchable from our website. Different antibodies against the same target such as Pcdhgb2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.