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anti-Pcdhgc3 antibody product blog

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Posted on 2015-09-16 10:45:42 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-PCDHGC3 antibody; PCDHGC3; Protocadherin Gamma C3;
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The Pcdhgc3 pcdhgc3 (Catalog #MBS803279) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Protocadherin Gamma C3 Antibody: ATTO 680 reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Protocadherin Gamma C3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
1:1000 WB. Researchers should empirically determine the suitability of the Pcdhgc3 pcdhgc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Pcdhgc3 pcdhgc3 product has the following accession number(s) (GI #18087747) (NCBI Accession #NP_291059.1) (Uniprot Accession #Q91XX1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Protocadherin Gamma C3 Antibody: ATTO 680 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3 showing detection of ~100 kDa Protocadherin Gamma C3 protein using Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody, Clone S174B-27. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.)
Western Blot (WB) Pcdhgc3.

Background Info: Detects a 100 kDa protein.

Scientific Background: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. PCDHGC3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

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