anti-PCNA antibody product blog
Tags: Antibody; PCNA; Monoclonal Antibody; anti-PCNA antibody;
The PCNA pcna (Catalog #MBS475120) is an Antibody produced from MouseSource: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-PCNA Mouse mAb reacts with Human, Mouse, Monkey, Rat, Hamster and may cross-react with other species as described in the data sheet. MyBioSource\'s PCNA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC).
WB: 1:1000
ICC: 1:100. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PCNA pcna product has the following accession number(s) (GI #4505641) (NCBI Accession #NP_002583.1) (Uniprot Accession #P12004). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-PCNA Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of PCNA in Hela, 3T3, COS7, C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.)
Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells using PCNA mouse mAb (dilution 1:100).Fixed in 100% methanol for 2hr at -20 degree C.)
Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using PCNA mouse mAb.)
Entrez Summary: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.[provided by RefSeq, Jul 2008].
Immunogen: Purified recombinant human PCNA protein fragments expressed in E Coli.
Sensitivity: This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.
Sequence Similarities: Belongs to the PCNA family. Subunit Structure: Homotrimer by similarity.Forms a complex with activator 1 heteropentamer in the presence of ATP.Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2.Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA.Forms a ternary complex with DNTTIP2 and core histone.Interacts with KCTD10 and PPP1R15A by similarity.Interacts with POLD1, POLD3 and POLD4.Interacts with BAZ1B; the interaction is direct.Interacts with HLTF and SHPRH.Interacts with NUDT15.Interaction is disrupted in response to UV irradiation and acetylation.Interacts with CDKN1A/p21 (CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX (DTL) complex.Interacts with DDX11.Interacts with EGFR; positively regulates PCNA.Interacts with PARPBP.Interacts (when ubiquitinated) with SPRTN; leading to enhance RAD18-mediated PCNA ubiquitination.Interacts (when polyubiquitinated) with ZRANB3.Interacts with SMARCAD1.Interacts with CDKN1C.Interacts with KIAA0101/PAF15 (via PIP-box).
Subcellular Location: Nucleus.