anti-PD-1/CD279 antibody product blog
Tags: Antibody; Monoclonal Antibody; PD-1/CD279; anti-PD-1/CD279 antibody;
The PD-1/CD279 pdcd1 (Catalog #MBS2542021) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PD-1/CD279 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s PD-1/CD279 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA).WB: 1:500-1:5000
IHC: 1:100-1:400
IF: 1:50-1:500. Researchers should empirically determine the suitability of the PD-1/CD279 pdcd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PD-1/CD279 pdcd1 product has the following accession number(s) (GI #167857792) (NCBI Accession #NP_005009.2) (Uniprot Accession #Q15116). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PD-1/CD279 Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Raji cells using PD-1/CD279 Monoclonal Antibody at dilution of 1:1000)
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity. The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE.
Immunogen: Fusion protein of PD-1/CD279. Buffer: PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation: Unconjugated. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PD-1/CD279 are readily searchable from our website. Different antibodies against the same target such as PD-1/CD279 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.