anti-PDCD1 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-PDCD1 antibody; PDCD1; PD-1;
The PDCD1 pdcd1 (Catalog #MBS154529) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PD-1 Antibody [8A4] reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PD-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF).Western blot at 0.5 - 1 mug/mL
Immunohistochemistry starting at 5 mug/mL.
The PDCD1 pdcd1 product has the following accession number(s) (GI #145559515) (NCBI Accession #NP_005009) (Uniprot Accession #Q15116). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PD-1 Antibody [8A4] with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of PD-1 in transfected 293 cell lysate with PD-1 antibody at (A) 0.5 and (B) 1 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of PD-1 in (A) human tonsil tissue, (B) human lymph node tissue, and (C) human spleen tissue with PD-1 antibody at 5 μg/mL. (D) Immunohistochemistry in human tonsil tissue with control mouse IgG staining at 5 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of PD-1 in (A) human breast cancer tissue and (B) human normal breast tissue with PD-1 antibody at 5 μg/mL.)
Immunocytochemistry (ICC) (Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunofluorescence in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.
Red: PD1 Antibody [8A4]
Blue: DAPI staining)
Western Blot (WB) (Western blot analysis of PD-1 in overexpressing 293 cells using antibody at 1, 0.5, and 0.25 μg/ml, respectively.)
ELISA (EIA) (Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA abs at decreasing concentrations.)
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.
Conjugate: Unconjugated
Immunogen: PD-1 antibody was raised against the extracellular domain of human PD-1.
Buffer: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide. Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PDCD1 are readily searchable from our website. Different antibodies against the same target such as PDCD1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Kidney tissues are correlated with this protein. The following patways have been known to be associated with this gene. Adenocarcinoma, Inflammation, Kidney Diseases, Liver Diseases, Liver Neoplasms, Lung Diseases, Lung Neoplasms, Lupus Erythematosus, Systemic, Melanoma, Necrosis are some of the diseases may be linked to PD-1 Antibody [8A4]. PDCD1 also interacts with the following gene(s): CD274, CD4, HLA-DR4, PDCD1LG2, PTPN11, PTPN6.