anti-PDCL3 antibody product blog
Tags: Antibody; Monoclonal Antibody; PDCL3; anti-PDCL3 antibody;
The PDCL3 pdcl3 (Catalog #MBS200313) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PDCL3 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PDCL3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the PDCL3 pdcl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PDCL3 pdcl3 product has the following accession number(s) (GI #13129044) (NCBI Accession #NP_076970) (Uniprot Accession #Q9H2J4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PDCL3 antibody with the following immunoassay(s):
Western Blot (WB) (Fig. 1: The extract of Jurkat (40ug), A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig. 2: The Human Recombinant protein PDCL3 (100ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF) (ICC/IF analysis of PDCL3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDCL3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of PDCL3 in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDCL3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
PDCL3 (phosducin-like 3), also known as VIAF1 (viral IAP-associated factor 1), VIAF, PHLP3 or HTPHLP, is a widely expressed protein that belongs to the phosducin-like family of proteins. phosducin-like family members associate with the cytosolic chaperonin complex (CCT) and regulate the folding of proteins. Localizing to the cytoplasm, PDCL3 does not appear to play a role in G protein signaling, but participates in the chaperone-assisted folding of proteins, such as beta Tubulin and Actin, that are involved in the regulation of cell cycle progression. More specifically, PDCL3, when associated with CCT, represses the ATPase activity of CCT and ultimately disrupts the folding of the Actin or tubulin substrates.
Antigen Species: Human
Immunogen: Recombinant human PDCL3 (1-239aa) purified from E. coli. Clone Note: Anti-human PDCL3 mAb, clone AT8F9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDCL3 protein.