anti-PEG antibody product blog
Tags: Antibody; Monoclonal Antibody; PEG; anti-PEG antibody;
The PEG n/a (Catalog #MBS840032) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-PEG Antibody (Clone 26A04) reacts with All. It reacts with PEGylated proteins such as PEG-GCSF, PEG-Erythropoietin, and PEG-Interferon, but does not react to unmodified form of the same proteins. and may cross-react with other species as described in the data sheet. MyBioSource\'s PEG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Serves as Positive Controls for Drmug Detection Assays. Researchers should empirically determine the suitability of the PEG n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-PEG Antibody (Clone 26A04) with the following immunoassay(s):
Testing Data (Lane 1: MW Marker
Lane 2: PEGylated protein)
Testing Data (An Indirect ELISA was run against a panel of three PEG conjugates and one unconjugated PEG (20 kDa). The PEG reagents were coated at 5 �g/ml and the antibodies were run at 2 �g/ml. The data shows that both MBS840003 and MBS840032 bind specifically to PEG in this application. Previous work also demonstrated that these antibodies are specific for the linear backbone of PEG and do not bind to the methoxy group.)
Polyethylene Glycol (PEG) is a polymer of ethylene oxide available in size variants from 400 Da to 40 kDa. It is nonionic, nontoxic, biocompatible, strong, and hydrophilic and has a large exclusion volume in aqueous solution. PEG has a number of industrial and biomedical applications. The modification of a biopharmaceutical with PEG increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. In particular, therapeutic proteins are conjugated with PEG to slow down their clearance from circulation and improve bioavailability. PEG antibodies can be a vital tool for propelling therapeutics to market by serving as a positive control anti-drug antibody, measuring clearance of a drug, or simply as a QA release confirming PEGylation.
Immunogen: PEG-KLH. Handling: The antibody solution should be gently mixed before use. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PEG are readily searchable from our website. Different antibodies against the same target such as PEG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.