anti-PGAM2 antibody product blog
Tags: Antibody; Monoclonal Antibody; PGAM2; anti-PGAM2 antibody;
The PGAM2 pgam2 (Catalog #MBS200398) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PGAM2 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PGAM2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the PGAM2 pgam2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The PGAM2 pgam2 product has the following accession number(s) (GI #50593010) (NCBI Accession #NP_000281) (Uniprot Accession #P15259). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PGAM2 antibody with the following immunoassay(s):
Western Blot (WB) (The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombnant protein (50ng) Lane 2. : HeLa cell lysate Lane 3. : HepG2 cell lysate Lane 4. : 293T cell lysate Lane 5. : Jurkat cell lysate Lane 6. : NIH3T3 cell lysate Lane 7. : A549 cell lysate Lane 8. : MCF7 cell lysate )
Immunofluorescence (IF) (ICC/IF analysis of PGAM2 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGAM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Since both 3-PGA and 2-PGA are allosteric regulators of the pentose phosphate pathway (PPP) and glycine and serine synthesis pathways, respectively, PGAM2 may contribute to the biosynthesis of amino acids, 5-carbon sugar, and nucleotides precursors. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this gene cause muscle phosphoglycerate mutase deficiency, also known as glycogen storage disease X. PGAM2 is one of two PGAM subunits found in humans and is predominantly expressed in adult muscle.
Antigen Species: Human
Immunogen: Recombinant human PGAM2 (1-253aa) purified from E. coli. Clone Note: Anti-human PGAM2 mAb, clone AT5A7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PGAM2 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PGAM2 are readily searchable from our website. Different antibodies against the same target such as PGAM2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.