anti-PHOSPHOTYROSINE antibody product blog
Tags: Antibody; Monoclonal Antibody; Phosphotyrosine; anti-Phosphotyrosine antibody;
The PHOSPHOTYROSINE n/a (Catalog #MBS212825) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s PHOSPHOTYROSINE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)*.ELISA: Maximum Dilution: 100ng/ml;
Western Blotting: Maximum Dilution: 2ug/ml; Application Note: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. Researchers should empirically determine the suitability of the PHOSPHOTYROSINE n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Mouse anti Phosphotyrosine antibody, clone PY20 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as an important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine. The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography. Buffer Solution: Borate buffered saline
Target Species: Chemical. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PHOSPHOTYROSINE are readily searchable from our website. Different antibodies against the same target such as PHOSPHOTYROSINE may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.