anti-Phosphotyrosine antibody product blog
Tags: Antibody; Monoclonal Antibody; Phosphotyrosine; anti-Phosphotyrosine antibody;
The Phosphotyrosine n/a (Catalog #MBS395126) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The phosphotyrosine rhodamine conjugate reacts with Ubituitous and may cross-react with other species as described in the data sheet. MyBioSource\'s Phosphotyrosine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA (EIA). Researchers should empirically determine the suitability of the Phosphotyrosine n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The rhodamine conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
Immunogen: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Phosphotyrosine are readily searchable from our website. Different antibodies against the same target such as Phosphotyrosine may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.