anti-PINK1 antibody product blog
Tags: Antibody; Monoclonal Antibody; PINK1; anti-PINK1 antibody;
The PINK1 pink1 (Catalog #MBS9200258) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PINK1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PINK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB).IHC~~1:50~100
IF~~1:25
WB~~1:100~500. Researchers should empirically determine the suitability of the PINK1 pink1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PINK1 pink1 product has the following accession number(s) (GI #14165272) (NCBI Accession #NP_115785.1) (Uniprot Accession #Q9BXM7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PINK1 Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. stomach section using Pink1(115-213). MBS9200258 was diluted at 1:25 dilution. An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. heart section using Pink1(115-213). MBS9200258 was diluted at 1:25 dilution. An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunofluorescence (IF) (Fluorescent image of PC12 cells stained with Pink1(115-213) Antibody. MBS9200258 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Western Blot (WB) (Western blot analysis of lysates from A431 cell line, mouse brain tissue lysate(from left to right), using Pink1 Antibody(115-213). MBS9200258 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB) (Western blot analysis of PINK (arrow) using mouse monoclonal PINK antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PINK gene (Lane 2) (Origene Technologies))
Immunohistochemistry (IHC) (PINK1 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINK1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.
Crown Antibody: Yes. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PINK1 are readily searchable from our website. Different antibodies against the same target such as PINK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.