|The PIR-A/B n/a (Catalog #MBS833866) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s PIR-A/B can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).
FC: 5 ul (0.5 ug)/test
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Researchers should empirically determine the suitability of the PIR-A/B n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PIR-A/B n/a product has the following accession number(s) (GI #6755064) (NCBI Accession #NP_035217.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PIR-A/B antibody (allophycocyanin) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of C57BL/6 splenocytes with CD19 antibody (FITC) and 0.125 ug of Rat IgG2b kappa Isotype Control (left) or 0.125 ug of PIR-A/B antibody (right). Total viable cells were used for analysis.)
Rat monoclonal PIR-A/B antibody (allophycocyanin).
Biological Significance: This 10-1-PIR monoclonal antibody reacts with mouse paired Ig-like receptors of activating -An and -B. These cell surface glycoproteins, which contain six extracellular Ig-like domains with distinct transmembrane and cytoplasmic regions, are expressed together on B lymphocytes, dendritic cells, macrophages, granulocytes, platelets, and mast cells. However, expression of PIR-A/B has not been observed on T cells, NK cells, and erythrocytes. The PIR-A receptor interacts with signaling molecules containing immunoreceptor tyrosine-based activation motifs which lead to its activating function. In contrast, the PIR-B receptor associates with proteins possessing immunoreceptor tyrosine-based inhibitory motifs therefore, this receptor has been shown to have an inhibitory function. PIRs bind MHC class I to modulate cell signaling and homeostasis of the immune system. Moreover, PIR-B knockout mice have been shown to exhibit susceptibility to Salmonella infection.Crossblocking studies indicate that 10-1-PIR recognizes a different epitope from 6C1. Tag/Conjugate: Allophycocyanin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PIR-A/B are readily searchable from our website. Different antibodies against the same target such as PIR-A/B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.