anti-Pirh2 antibody product blog
Tags: Antibody; Monoclonal Antibody; Pirh2; anti-Pirh2 antibody;
The Pirh2 rchy1 (Catalog #MBS850972) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Pirh2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Pirh2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA).WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000. Researchers should empirically determine the suitability of the Pirh2 rchy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Pirh2 rchy1 product has the following accession number(s) (GI #58331197) (NCBI Accession #NP_001008925.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Pirh2 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.)
Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb)
Flow Cytometry (FC/FACS) (Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).)
Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N-and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
Immunogen: Purified recombinant fragment of human Pirh2 expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Pirh2 are readily searchable from our website. Different antibodies against the same target such as Pirh2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Embryonic Tissue, Heart, Kidney, Liver, Lung, Lymph Node, Muscle, Prostate, Skin tissues are correlated with this protein. Pirh2 also interacts with the following gene(s): CDKN1B, KAT5, MDM2, TP53.