anti-POGK antibody product blog
Tags: Antibody; Monoclonal Antibody; POGK; anti-POGK antibody;
The POGK n/a (Catalog #MBS6012990) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The POGK (KIAA1513, Pogo Transposable Element with KRAB Domain, LST003, SLTP003) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s POGK can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF).Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the POGK n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The POGK n/a product has the following accession number(s) (GI #149642907) (NCBI Accession #NP_001092570.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MEIRCHRYGW MTEDLMQDWL EVVWRRRTGA VPKQRGMLIL NGFRGHATDS VKNSMESMNT DMVIIPGGLT SQLQVLDVVV YKPLNDSVRA QYSNWLLAGN LALSPTGNAK.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the POGK (KIAA1513, Pogo Transposable Element with KRAB Domain, LST003, SLTP003) with the following immunoassay(s):
Western Blot (WB) (Western Blot detection against Immunogen (38.21kD).)
Western Blot (WB) (POGK monoclonal antibody, Western Blot analysis of POGK expression in HeLa NE.)
Western Blot (WB) (Western Blot analysis of POGK expression in transfected 293T cell line by POGK monoclonal antibody.|Lane 1: POGK transfected lysate (69.4kD).|Lane 2: Non-transfected lysate.)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to POGK on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data (Detection limit for recombinant GST tagged POGK is ~1ng/ml as a capture antibody.)
The exact function of the protein encoded by this gene is not known. However, this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus, and a transposase domain at the C-terminus, suggesting that it may belong to the family of DNA-mediated transposons in human.
Immunogen: Partial recombinant corresponding to aa431-541 from human POGK (NP_060012) with GST tag. MW of the GST tag alone is 26kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing POGK are readily searchable from our website. Different antibodies against the same target such as POGK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.