anti-POLR2J antibody product blog
Tags: Antibody; Monoclonal Antibody; POLR2J; anti-POLR2J antibody;
The POLR2J polr2j (Catalog #MBS644045) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The POLR2J (POLR2J1, DNA-directed RNA Polymerase II Subunit RPB11-a, DNA-directed RNA Polymerase II Subunit J-1, RNA Polymerase II 13.3kD Subunit, MGC71910) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s POLR2J can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the POLR2J polr2j for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The POLR2J polr2j product has the following accession number(s) (GI #146327795) (NCBI Accession #AAI41831.1) (Uniprot Accession #P52435). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTNA ITDLISELSL LEERFRVAIK DKQEGIE.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the POLR2J (POLR2J1, DNA-directed RNA Polymerase II Subunit RPB11-a, DNA-directed RNA Polymerase II Subunit J-1, RNA Polymerase II 13.3kD Subunit, MGC71910) with the following immunoassay(s):
Western Blot (WB) (Western Blot detection against Immunogen (38.61kD).)
Western Blot (WB) (Western Blot analysis of POLR2J expression in transfected 293T cell line by POLR2J monoclonal antibody.|Lane 1: POLR2J transfected lysate (13.3kD).|Lane 2: Non-transfected lysate.)
Testing Data (Detection limit for recombinant GST tagged POLR2J is 0.3ng/ml as a capture antibody.)
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
Immunogen: Full length recombinant corresponding to aa1-118 from human POLR2J (AAH24165) with GST tag. MW of the GST tag alone is 26kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing POLR2J are readily searchable from our website. Different antibodies against the same target such as POLR2J may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.