|The Polymyxin B n/a (Catalog #MBS584369) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal antibody to Polymyxin B (PMB) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Polymyxin B can be used in a range of immunoassay formats including, but not limited to, Immunoassay (IA), Western Blot (WB).
Application Use: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Researchers should empirically determine the suitability of the Polymyxin B n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Product Description: The monoclonal antibody 45 reacts with Polymyxin B. The antibody binds to free Polymyxin B as well as to Polymyxin B already bound to LPS. The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Monoclonal antibody 45 enables the possibilities to study quantitatively the interaction of PMB and LPS.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Polymyxin B are readily searchable from our website. Different antibodies against the same target such as Polymyxin B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.