anti-POMC antibody product blog
Tags: Antibody; ACTH; Monoclonal Antibody; POMC; anti-POMC antibody;
The POMC pomc (Catalog #MBS438377) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACTH reacts with Human, Mouse, Rat. Expected to show a broad species reactivity. and may cross-react with other species as described in the data sheet. MyBioSource\'s ACTH can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC).ELISA (For coating, order Ab without BSA)
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunoprecipitation (1-2ug/500ug protein lysate)
Immunohistochemistry (Frozen & Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(No special pretreatment is required for staining of formalin-fixed tissues)
Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the POMC pomc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The POMC pomc product has the following accession number(s) (GI #4505949) (NCBI Accession #NP_000930.1) (Uniprot Accession #P01189). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ACTH with the following immunoassay(s):
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Human Pituitary stained with ACTHMonoclonal Antibody (SPM333).)
Localization: Cytoplasmic
Positive Control: Normal pituitary gland or pituitary tumor
Human Chromosomal Location: 2p23.3. Immunogen: Synthetic peptide corresponding to aa1-24 of human ACTH
Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing POMC are readily searchable from our website. Different antibodies against the same target such as POMC may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. POMC also interacts with the following gene(s): CRH, CYP17A1, GIPR, MC1R, MC2R, MC3R, MC4R, MC5R, NPY, OPRM1. Brain, Embryonic Tissue, Eye, Intestine, Lung, Pituitary Gland, Placenta, Testis, Uncharacterized Tissue, Uterus tissues are correlated with this protein. The following patways have been known to be associated with this gene. Cushing Syndrome, Diabetes Mellitus, Gastrointestinal Diseases, Hemorrhage, Immune System Diseases, Infection, Kidney Diseases, Mental Disorders, Necrosis, Obesity are some of the diseases may be linked to ACTH.