Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-PP5 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • anti-EDR antibody blog
 • anti-V5 Tag antibody blog
 • anti-IgG3 antibody blog
 • anti-UBB antibody blog
 • anti-mCherry antibody blog
 • anti-CCL11 antibody blog
 • anti-CD13 antibody blog
 • anti-Hsf1 antibody blog
 • anti-INHA antibody blog
 • anti-WASF1 antibody blog
 • anti-CEACAM5 antibody blog
 • anti-MKRN2 antibody blog
 • anti-NAP3 antibody blog
 • anti-B7RP1 antibody blog
 • anti-PDLIM2 antibody blog
 • anti-RUNX3 antibody blog
 • anti-TCF-4 antibody blog
 • anti-IL18 antibody blog
 • anti-EFNA2 antibody blog
 • anti-KDM1/LSD1 antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • beta-Hex B ELISA Kit
 • heterogeneous nuclear ribonucleoprotein P2 (hnRNP P2) ELISA Kit
 • Prohibitin (PHB) Antibody
 • AARS2 Antibody
 • TRIP13 Antibody
 • arylacetamide deacetylase-like 1 (NCEH1) Antibody
 • Protein A Biochemical
 • Collagen Type II Cleavage (C2C) ELISA Kit
 • Ankyrin Repeat Domain-containing Protein 12 (ANKRD12) ELISA Kit
 • Leukocyte immunoglobulin-like receptor subfamily B member 3 ... Recombinant Protein
 • Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ELISA Kit
 • Glucose Regulated Protein 94 (GRP94) ELISA Kit
 downarrow more ...

anti-PP5 antibody product blog

Posted on 2016-04-27 21:22:01 by mybiosource_staff
Share it on ...     
Tags: Antibody; Monoclonal Antibody; PP5; anti-PP5 antibody;
The PP5 ppp5c (Catalog #MBS807048) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human PP5 Monoclonal IgG1 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Western Blot: 1:1000
Optimal dilutions should be determined by the user. Researchers should empirically determine the suitability of the PP5 ppp5c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PP5 ppp5c product has the following accession number(s) (GI #5453958) (NCBI Accession #NP_006238.1) (Uniprot Accession #P53041). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Anti-Human PP5 Monoclonal IgG1 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Lane 1: MW Ladder. Lane 2: Human A431 (15 ug). Lane 3: Human HEK293 (15 ug). Lane 4: Human Jurkat (15 ug). Load: 15 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.)
Western Blot (WB) PP5.

Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues, potentially playing a role in the regulation of cell growth and differentiation. PP5 or PPT is predominantly a nuclear protein and interacts with CDC16 and CDC27. It is found in association with several proteins that influence intracellular signlaing-cascades intitiated by hormones (glucocorticoids) or cellular stress (hypoxia, oxidative stress and DNA Damage). It phosphorylates serine residues of skeletal muscle phorylatse and histone H1. It may also be involved in mitosis and RNA biogenesis regulation (1,2).

Immunogen: Full length human PP5 protein
Immunogen Species: Human. COA: 1 ug/ml was sufficient for detection of PP5 in 15 ug of human A431 lysates by ECL analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
Conjugate: ATTO 680
Cellular Localization: Nucleus, Cytoplasm. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PP5 are readily searchable from our website. Different antibodies against the same target such as PP5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions