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anti-PRKAA1 antibody product blog

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Posted on 2015-10-23 16:22:08 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-PRKAA1 antibody; PRKAA1;
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The PRKAA1 prkaa1 (Catalog #MBS850198) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRKAA1 Antibody reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s PRKAA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA).
WB: 1/500 - 1/2000, IHC: 1/200-1/1000, IF: 1/200-1/1000, FC: 1/200-1/400, ELISA: Propose dilution 1/10000
Not yet tested in other applications. Researchers should empirically determine the suitability of the PRKAA1 prkaa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PRKAA1 prkaa1 product has the following accession number(s) (GI #94557301) (NCBI Accession #NP_006242.5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PRKAA1 Antibody with the following immunoassay(s):
Testing Data (Figure 1: Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.)
Testing Data PRKAA1.

Testing Data #2 (Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.)
Testing Data #2 PRKAA1.

Testing Data #3
Testing Data #3 PRKAA1.

Background: The protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5\'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen: Purified recombinant fragment of human PRKAA1 expressed in E. Coli.
Species: Mouse IgG1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PRKAA1 are readily searchable from our website. Different antibodies against the same target such as PRKAA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. PRKAA1 also interacts with the following gene(s): PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3, STK11, TSC2. Adipose Tissue, Blood, Brain, Connective Tissue, Liver, Lung, Muscle, Nerve, Pancreas, Vascular tissues are correlated with this protein.
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