anti-PRKCE antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-PRKCE antibody; PRKCE;
The PRKCE prkce (Catalog #MBS837971) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s PRKCE can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB).IF: 1:100
IHC: 1:150
WB: 1:1000-2000. Researchers should empirically determine the suitability of the PRKCE prkce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PRKCE prkce product has the following accession number(s) (GI #4885563) (NCBI Accession #NP_005391.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PRKCE antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PRKCE protein (Right) or empty vector (Left) detected with PRKCE antibody)
Immunohistochemistry (IHC) (Immunohistochemical analysis of PRKCE protein in paraffin embedded Adenocarcinoma of Human colon tissue using PRKCE antibody)
Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PRKCE protein using PRKCE antibody)
Mouse monoclonal PRKCE antibody.
Biological Significance: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calciumand the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and areknown to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. this kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Cross-Reactivity: Human, Dog, Monkey
Immunogen: PRKCE antibody was raised in mouse using a full length recombinant protein of human PRKCE (NP_005391) produced in HEK293T cells, as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PRKCE are readily searchable from our website. Different antibodies against the same target such as PRKCE may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.