anti-PRKD3 antibody product blog
Tags: Antibody; Monoclonal Antibody; PRKD3; anti-PRKD3 antibody;
The PRKD3 prkd3 (Catalog #MBS646820) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRKD3 (Serine/Threonine-protein Kinase D3, Protein Kinase C nu Type, Protein Kinase EPK2, nPKC-nu, EPK2, PRKCN) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PRKD3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the PRKD3 prkd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PRKD3 prkd3 product has the following accession number(s) (GI #22658307) (NCBI Accession #AAH30706.1) (Uniprot Accession #O94806). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MSANNSPPSA QKSVLPTAIP AVLPAASPCS SPKTGLSARL SNGSFSAPSL TNSRGSVHTV SFLLQIGLTR ESVTIEAQEL SLSAVKDLVC SIVYQKFPEC.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PRKD3 (Serine/Threonine-protein Kinase D3, Protein Kinase C nu Type, Protein Kinase EPK2, nPKC-nu, EPK2, PRKCN) with the following immunoassay(s):
Western Blot (WB) (Western Blot detection against Immunogen (37kD).)
Testing Data (Detection limit for recombinant GST tagged PRKD3 is ~1ng/ml as a capture antibody.)
Protein kinase D (PKD), a serine/threonine kinase originally described as a novel PKC family member and termed PKCm, belongs to the calcium calmodulin superfamily of kinases. Three mammalian isoforms have so far been described - PKD1/PKCm, PKD2 and PKD3/PKCn; these isoforms show a high degree of homology, especially in their catalytic domain. PKDs are major targets for tumor promoting phorbol esters; they are activated via G protein-coupled receptors (GPCRs) and their activation is also dependent on PKC activation. PKDs have been implicated in numerous cellular functions, including signal transduction as well as cell survival, migration, differenciation, and proliferation. They are important regulators of secretary transport at the trans- golgi network. Of the three isoforms, PKD1 is the best characterized. It is involved in the regulation of Golgi function, cell proliferation and apoptosis and it mediates oxidative stress signaling regulating cellular detoxification and survival. PKD2 has been found to phosphorylate histone H1 more efficiently than aldolase in vitro. PKD3 plays a role in the formation of vesicular transport carriers at the trans-Golgi network (TGN) and in basal glucose transport in vitro studies.
Immunogen: Partial recombinant corresponding to aa1-100 from PRKD3 (AAH30706) with GST tag. MW of the GST tag alone is 26kD.