anti-PRKDC antibody product blog
Tags: Antibody; PRKDC; Monoclonal Antibody; anti-PRKDC antibody; DNA-PKcs;
The PRKDC prkdc (Catalog #MBS9215601) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DNA-PKcs Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DNA-PKcs can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP).WB ~~ 1:1000
ICC ~~ 1:200
IP ~~ 1:500. Researchers should empirically determine the suitability of the PRKDC prkdc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PRKDC prkdc product has the following accession number(s) (GI #126032350) (NCBI Accession #NP_001075109.1) (Uniprot Accession #P78527). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DNA-PKcs Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot detection of DNA-PKcs in Hela,K562,HL-60 and MOLT-4 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.)
Immunocytochemistry (ICC) (Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:200).)
Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using DNA-PKcs mouse mAb.)
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates \'Ser-139\' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 \'Ser-588\' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.