anti-PSMC3 antibody product blog
Tags: Antibody; Monoclonal Antibody; PSMC3; anti-PSMC3 antibody;
The PSMC3 psmc3 (Catalog #MBS838262) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s PSMC3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB).FC: 1:100
IF: 1:100
IHC: 1:50
WB: 1:2000. Researchers should empirically determine the suitability of the PSMC3 psmc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PSMC3 psmc3 product has the following accession number(s) (GI #48145579) (NCBI Accession #CAG33012.1) (Uniprot Accession #P17980). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PSMC3 antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PSMC3 protein (Right) or empty vector (Left) detected with PSMC3 antibody)
Immunohistochemistry (IHC) (Immunohistochemical analysis of PSMC3 protein in paraffin embedded Human kidney tissue using PSMC3 antibody)
Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PSMC3 protein using PSMC3 antibody)
Mouse monoclonal PSMC3 antibody.
Biological Significance: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composedof 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in anATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. Cross-Reactivity: Human
Immunogen: PSMC3 antibody was raised in mouse using a full length recombinant protein of human PSMC3 (NP_002795) produced in HEK293T cells, as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PSMC3 are readily searchable from our website. Different antibodies against the same target such as PSMC3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.