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anti-PTCH1 antibody product blog

Posted on 2015-10-27 14:19:27 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-PTCH1 antibody; PTCH1;
The PTCH1 ptch1 (Catalog #MBS832597) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PTCH1 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PTCH1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB).
IF: 1:100
IHC: 1:50
WB: 1:500. Researchers should empirically determine the suitability of the PTCH1 ptch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PTCH1 ptch1 product has the following accession number(s) (GI #134254446) (NCBI Accession #NP_000255) (Uniprot Accession #Q13635). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PTCH1 antibody with the following immunoassay(s):
Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant PTCH1 protein using PTCH1 antibody)
Immunofluorescence (IF) PTCH1.

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PTCH1 protein (Right) or empty vector (Left) detected with PTCH1 antibody)
Western Blot (WB) PTCH1.

Immunohistochemistry (IHC) (Immunohistochemical analysis of PTCH1 protein in paraffin embedded Human Kidney tissue using PTCH1 antibody)
Immunohistochemistry (IHC) PTCH1.

Mouse monoclonal PTCH1 antibody.

Biological Significance: Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas. Immunogen: PTCH1 antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 122-436 of human PTCH1 (NP_000255) produced in E Coli as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PTCH1 are readily searchable from our website. Different antibodies against the same target such as PTCH1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Brain, Connective Tissue, Embryonic Tissue, Eye, Lung, Mouth, Muscle, Prostate, Skin, Stomach tissues are correlated with this protein. Basal Cell Nevus Syndrome, Brain Neoplasms, Carcinoma, Basal Cell, Cell Transformation, Neoplastic, Disease Models, Animal, Medulloblastoma, Neoplasms, Experimental, Neuroectodermal Tumors, Primitive, Rhabdomyosarcoma, Skin Neoplasms are some of the diseases may be linked to PTCH1 antibody.
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