anti-PTEN antibody product blog
Tags: Antibody; PTEN; Monoclonal Antibody; anti-PTEN antibody;
The PTEN pten (Catalog #MBS9230950) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PTEN Antibody (N-term) reacts with Human, Mouse, RatPredicted: Xenopus and may cross-react with other species as described in the data sheet. MyBioSource\'s PTEN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).
WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PTEN pten for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PTEN pten product has the following accession number(s) (GI #73765544) (NCBI Accession #NP_000305.3) (Uniprot Accession #P60484). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PTEN Antibody (N-term) with the following immunoassay(s):
Western Blot (WB) (All lanes : Anti-PTEN Antibody (N-term) at 1:500-1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: C6 whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 47 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine-and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns (3,4,5)P3 > PtdIns (3,4)P2 > PtdIns3P > Ins (1,3,4,5)P4 (PubMed:26504226). The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.
Antigen Type: Synthesized Peptide
Antigen Source: Human. Cellular Location: Cytoplasm. Nucleus. Nucleus, PML body. Note=Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies XIAP/BIRC4 promotes its nuclear localization
Tissue Location: Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.