anti-RAD51 antibody product blog
Tags: Antibody; Monoclonal Antibody; Rad51; anti-RAD51 antibody;
The Rad51 rad51 (Catalog #MBS475181) is an Antibody produced from MouseSource: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rad51 Mouse mAb reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s Rad51 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
WB: 1:1000. Researchers should empirically determine the suitability of the Rad51 rad51 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Rad51 rad51 product has the following accession number(s) (GI #256017143) (NCBI Accession #NP_001157741.1) (Uniprot Accession #Q06609). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Rad51 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of Rad51 in CHO-K1, COS7, C6, 3T3 and Hela cell lysates using Rad51 mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:37KDa.)
Entrez Summary: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen: Recombinant protein of human Rad51.
Sensitivity: This antibody detects endogenous levels of Rad51 and does not cross-react with related proteins.
Sequence Similarities: Belongs to the RecA family. RAD51 subfamily. Contains 1 HhH domain. Subunit Structure: Forms linear homooligomers, giving rise to a RAD51 nucleoprotein filament, which is essential for strand-pairing reactions during DNA recombination. Interacts with BRCA1 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Interacts with the BCDX2 subcomplex RAD51C:RAD51B. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with RAD51AP1 and RAD51AP2. Interacts with CHEK1, and this may require prior phosphorylation of CHEK1. Interacts with the MND1-PSMC3IP heterodimer. Found in a complex, at least composed of BLM, RAD51 and SPIDR; the complex formation is mediated by SPIDR. Interacts with SPIDR; the interaction is direct and recruits RAD51 to DNA damage sites. Interacts with FIGNL1 (via N-terminal one-half region); the interaction is direct. Interacts with RAD51AP1 (via C-terminal region); the interaction is direct. Interacts with NABP2, RPA1, PALB2 and RAD51. Interacts with SWI5/C9orf119, and at lower level with SFR1/MEIR5. Interacts with hyperphosphorylated RPA2; this interaction is necessary for efficient recruitment to chromatin in response to DNA damage. Interacts with SWSAP1; involved in homologous recombination repair. Interacts with PARPBP, BRCA2 and RECQL5; these interactions interfere with the formation of the RAD51-DNA homologous recombination structure.
Subcellular Location: Nucleus. Cytoplasm. Cytoplasm; perinuclear region. Mitochondrion matrix. Cytoplasm; cytoskeleton; microtubule organizing center; centrosome.